02544nas a2200265   4500008004100000245009800041210006900139260001100208300001200219490000800231520180300239653001302042653000902055653002202064653001402086653002202100653001402122653001802136653001002154653001402164653001702178100002102195700002102216856004102237       2004                        eng d00aPerformance of maximum parsimony and likelihood phylogenetics when evolution is heterogeneous0 aPerformance of maximum parsimony and likelihood phylogenetics wh  cOCT 21  a980-9840 v4313 aAll inferences in comparative biology depend on accurate estimates of evolutionary relationships. Recent phylogenetic analyses have turned away from maximum parsimony towards the probabilistic techniques of maximum likelihood and bayesian Markov chain Monte Carlo (BMCMC). These probabilistic techniques represent a parametric approach to statistical phylogenetics, because their criterion for evaluating a topology-the probability of the data, given the tree-is calculated with reference to an explicit evolutionary model from which the data are assumed to be identically distributed. Maximum parsimony can be considered nonparametric, because trees are evaluated on the basis of a general metric-the minimum number of character state changes required to generate the data on a given tree-without assuming a specific distribution(1). The shift to parametric methods was spurred, in large part, by studies showing that although both approaches perform well most of the time(2), maximum parsimony is strongly biased towards recovering an incorrect tree under certain combinations of branch lengths, whereas maximum likelihood is not(3-6). All these evaluations simulated sequences by a largely homogeneous evolutionary process in which data are identically distributed. There is ample evidence, however, that real-world gene sequences evolve heterogeneously and are not identically distributed(7-16). Here we show that maximum likelihood and BMCMC can become strongly biased and statistically inconsistent when the rates at which sequence sites evolve change non-identically over time. Maximum parsimony performs substantially better than current parametric methods over a wide range of conditions tested, including moderate heterogeneity and phylogenetic problems not normally considered difficult.10aaccuracy10abias10acovariotide model10ainference10amitochondrial-DNA10amolecules10aphylogenetics10arates10asequences10asubstitution1 aKolaczkowski, B.1 aThornton, J., W.  u<u><Go to ISI>://000224585600043</u>