02784nas a2200229   4500008004100000245012900041210006900170260001400239300001200253490000800265520200700273653001302280653001202293653001302305653002702318653001402345653001502359100003002374700002602404700002402430856010002454       2006                        eng d00aThe nutritional value of seven species of tropical microalgae for black-lip pearl oyster (Pinctada margaritifera, L.) larvae0 anutritional value of seven species of tropical microalgae for bl  c2006/6/30  a491-5030 v2573 aRecent years have seen major developments in the culture and availability of tropical microalgae as a food source for tropical bivalve species. The nutritional value of seven small (Chaetoceros muelleri and Chaetoceros sp.); three golden-brown flagellates (Isochrysis sp., Pavlova salina and Pavlova sp.) and two green-flagellates (Micromonas pusilla and an unidentified coccoid CS-126), were analysed for carbohydrate, lipid and protein contents as well as fatty acid composition. Each species of microalgae was fed singly to early (D-stage veliger) and later (umbo stage veliger) stage larvae of the black-lip pearl oyster, Pinctada margaritifera. Highest survival of D-stage larvae over the 10-day experiment was recorded for those fed Pavlova sp. (CS-50). Greatest shell growth was shown by D-stage larvae fed the golden-flagellates Pavlova sp. (CS-50) and Pav. salina. Based on growth of D-stage larvae, the microalgae could be divided into three groups: (1) larvae fed Pav. salina and Pavlova sp. showed significantly greater growth than those fed other microalgae; (2) those fed Isochrysis sp., C. muelleri and M. pusilla showed significantly greater growth than unfed larvae; and (3) larvae fed Chaetoceros sp. and CS-126 did not grow at a rate greater than unfed larvae. Growth of D-stage veliger larvae was significantly correlated with carbohydrate, lipid and protein content of microalgae and with levels of dietary polyunsaturated fatty acid, specifically DHA (r = 0.829, P = 0.021). In a second experiment survival of umbo-stage larvae (including the unfed control) did not differ significantly between treatments (P Pavlova sp. and Pav. salina showed the greatest incremental growth increases, but these were not significantly greater than those of larvae fed TISO and C. muelleri (P > 0.05). Growth of umbo-stage larvae fed M. pusilla, Chaetoceros sp. and the Prasinophyta sp. (CS-126) did not differ significantly from that of unfed larvae (P P. margaritifera larvae of different ages.10abivalvia10aecology10amollusca10apinctada margaritifera10apteriidae10apterioidea1 aMartinez-Fernandez, Erika1 aAcosta-Salmon, Hector1 aSouthgate, Paul, C.  uhttp://www.sciencedirect.com/science/article/B6T4D-4JJFV6W-1/2/11068a6cabf92db1db1a519f1f3fe91402755nas a2200289   4500008004100000245010200041210006900143260001400212300001200226490000800238520188400246653001202130653001302142653001102155653001302166653001802179653001502197653001302212653002002225653002702245653001402272653001402286653001502300100002602315700002402341856010002365       2005                        eng d00aMantle regeneration in the pearl oysters <i>Pinctada fucata</i> and <i>Pinctada margaritifera</i>0 aMantle regeneration in the pearl oysters iPinctada fucatai and i  c2005/5/18  a447-4530 v2463 aMantle tissue in pearl oysters (Pteriidae) is responsible for secreting the mother-of-pearl or nacre lining the shell. When grafted into another oyster, excised mantle tissue, commonly called 'saibo', is responsible for cultured pearl production. This study was undertaken to assess the process of mantle regeneration in the Akoya pearl oyster Pinctada fucata and the blacklip pearl oyster Pinctada margaritifera following mantle excision. Prior to saibo excision, oysters were anaesthetized with 2 mL L-1 propylene phenoxetol. Saibo tissue was excised from 50 P. fucata and 10 P. margaritifera. After excision, all oysters were returned to culture conditions. All oysters were maintained in panel (pocket) nets on a long-line at Magnetic Island, north Queensland, Australia. Oysters were anaesthetized and sacrificed 3, 6, 9, 12, 15, 20, 30, 45, 60 and 90 days after saibo excision to assess mantle regeneration using histological and histochemical techniques. Survival over the 90-day study was 100% and 70% for P. fucata and P. margaritifera, respectively. After excision, the mantle tissue healed within the first three days and began growing as connective tissue. Muscular development was seen between days 60 and 90 after excision. Shell formation abilities were recovered by day 15 when secretory cells and conchiolin secretions were first observed. Both P. fucata and P. margaritifera regenerated mantle to its original extent within the 90-day study. This is the first description of in vivo mantle regeneration in pearl oysters and the results have major implications for the lucrative cultured pearl industry. Our results indicate that mantle tissue donors need not be killed for pearl production. Those producing saibo that results in good quality pearls could be used as future parent-stock in breeding programs or for subsequent saibo donation and pearl production.10aanatomy10abivalvia10amantle10amollusca10apearliculture10aphysiology10apinctada10apinctada fucata10apinctada margaritifera10apteriidae10apterioida10apterioidea1 aAcosta-Salmon, Hector1 aSouthgate, Paul, C.  uhttp://www.sciencedirect.com/science/article/B6T4D-4FNP2DM-7/2/e1a4fa830d61f99d0d8c755b5419d1d002715nas a2200277   4500008004100000245008800041210006900129300001200198490000800210520188400218653001202102653001302114653001102127653001302138653001802151653001502169653001302184653002002197653002702217653001402244653001402258653001502272100002602287700002402313856010002337       2005                        eng d00aMantle regeneration in the pearl oysters Pinctada fucata and Pinctada margaritifera0 aMantle regeneration in the pearl oysters Pinctada fucata and Pin  a447-4530 v2463 aMantle tissue in pearl oysters (Pteriidae) is responsible for secreting the mother-of-pearl or nacre lining the shell. When grafted into another oyster, excised mantle tissue, commonly called 'saibo', is responsible for cultured pearl production. This study was undertaken to assess the process of mantle regeneration in the Akoya pearl oyster Pinctada fucata and the blacklip pearl oyster Pinctada margaritifera following mantle excision. Prior to saibo excision, oysters were anaesthetized with 2 mL L-1 propylene phenoxetol. Saibo tissue was excised from 50 P. fucata and 10 P. margaritifera. After excision, all oysters were returned to culture conditions. All oysters were maintained in panel (pocket) nets on a long-line at Magnetic Island, north Queensland, Australia. Oysters were anaesthetized and sacrificed 3, 6, 9, 12, 15, 20, 30, 45, 60 and 90 days after saibo excision to assess mantle regeneration using histological and histochemical techniques. Survival over the 90-day study was 100% and 70% for P. fucata and P. margaritifera, respectively. After excision, the mantle tissue healed within the first three days and began growing as connective tissue. Muscular development was seen between days 60 and 90 after excision. Shell formation abilities were recovered by day 15 when secretory cells and conchiolin secretions were first observed. Both P. fucata and P. margaritifera regenerated mantle to its original extent within the 90-day study. This is the first description of in vivo mantle regeneration in pearl oysters and the results have major implications for the lucrative cultured pearl industry. Our results indicate that mantle tissue donors need not be killed for pearl production. Those producing saibo that results in good quality pearls could be used as future parent-stock in breeding programs or for subsequent saibo donation and pearl production.10aanatomy10abivalvia10amantle10amollusca10apearliculture10aphysiology10apinctada10apinctada fucata10apinctada margaritifera10apteriidae10apterioida10apterioidea1 aAcosta-Salmon, Hector1 aSouthgate, Paul, C.  uhttp://www.sciencedirect.com/science/article/B6T4D-4FNP2DM-7/2/e1a4fa830d61f99d0d8c755b5419d1d002728nas a2200193   4500008004100000245012900041210006900170300001200239490000800251520200700259653001302266653001302279653004702292653001502339100003002354700002602384700002402410856010002434       2006                        eng d00aThe nutritional value of seven species of tropical microalgae for black-lip pearl oyster (Pinctada margaritifera, L.) larvae0 anutritional value of seven species of tropical microalgae for bl  a491-5030 v2573 aRecent years have seen major developments in the culture and availability of tropical microalgae as a food source for tropical bivalve species. The nutritional value of seven small (Chaetoceros muelleri and Chaetoceros sp.); three golden-brown flagellates (Isochrysis sp., Pavlova salina and Pavlova sp.) and two green-flagellates (Micromonas pusilla and an unidentified coccoid CS-126), were analysed for carbohydrate, lipid and protein contents as well as fatty acid composition. Each species of microalgae was fed singly to early (D-stage veliger) and later (umbo stage veliger) stage larvae of the black-lip pearl oyster, Pinctada margaritifera. Highest survival of D-stage larvae over the 10-day experiment was recorded for those fed Pavlova sp. (CS-50). Greatest shell growth was shown by D-stage larvae fed the golden-flagellates Pavlova sp. (CS-50) and Pav. salina. Based on growth of D-stage larvae, the microalgae could be divided into three groups: (1) larvae fed Pav. salina and Pavlova sp. showed significantly greater growth than those fed other microalgae; (2) those fed Isochrysis sp., C. muelleri and M. pusilla showed significantly greater growth than unfed larvae; and (3) larvae fed Chaetoceros sp. and CS-126 did not grow at a rate greater than unfed larvae. Growth of D-stage veliger larvae was significantly correlated with carbohydrate, lipid and protein content of microalgae and with levels of dietary polyunsaturated fatty acid, specifically DHA (r = 0.829, P = 0.021). In a second experiment survival of umbo-stage larvae (including the unfed control) did not differ significantly between treatments (P Pavlova sp. and Pav. salina showed the greatest incremental growth increases, but these were not significantly greater than those of larvae fed TISO and C. muelleri (P > 0.05). Growth of umbo-stage larvae fed M. pusilla, Chaetoceros sp. and the Prasinophyta sp. (CS-126) did not differ significantly from that of unfed larvae (P P. margaritifera larvae of different ages.10abivalvia10amollusca10apteriidae, Pinctada margaritifera, ecology10apterioidea1 aMartinez-Fernandez, Erika1 aAcosta-Salmon, Hector1 aSouthgate, Paul, C.  uhttp://www.sciencedirect.com/science/article/B6T4D-4JJFV6W-1/2/11068a6cabf92db1db1a519f1f3fe914